If the unknown antigen is Neisseria gonorrhoeae, the known antibodies against N. gonorrhoeae with attached fluorescent dye will bind to the bacterium and will not wash off. The bacteria will fluoresce when viewed under a fluorescent microscope.

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Microbiology Laboratory Manual by Gary E. Kaiser, PhD, Professor of Microbiology
is licensed under a Creative Commons Attribution 4.0 International License.
Last updated: September, 2017